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投稿时间：2019-06-04
投稿时间：2019-06-04
中文摘要: 牛血清白蛋白（bovine serum albumin，BSA）可通过糖基化反应与葡聚糖（dextran）形成复合物，可作为油溶性营养素的乳化包埋载体。以乳化活性为指标，通过单因素试验以及响应面试验，优化湿法美拉德反应制备BSA-dextran复合物的最佳工艺条件。研究结果表明：复合物最佳工艺条件为BSA浓度2.78 mg/mL、dextran∶BSA（摩尔比）=6.89∶1、pH 8.50、反应时间 2.3 h、反应温度 95.0℃；所得BSA-dextran乳化活性为（31.743±0.45）m2/g；该复合物对叶黄素包埋的包封率为95.86%，明显高于BSA对叶黄素包埋的包封率（77.82%）。
Abstract:Through glycosylation reaction，bovine serum albumin （BSA）and dextran can form conjugate，which can be used as an emulsifying carrier for oil-soluble nutrients.In this study，taking emulsifying activity as evaluation index，the optimal process preparation conditions of BSA-dextran conjugate through wet Maillard reaction were assured by single factor experiments and response surface experiments.Results showed that：the best preparation process of BSA-dextran conjugate was BSA concentration 2.78 mg/mL，the molar mass ratio of dextran to BSA 6.89∶1，pH of buffer 8.5，the reaction time 2.3 h，and the reaction temperature 95℃；the emulsifying activity of the conjugate was（31.743±0.45）m2/g.The encapsulation efficiency of the conjugate to lutein was 95.86%，which was significantly higher than that of BSA（77.82%）.
keywords: bovine serum albumin dextran wet Maillard reaction response surface method emulsifying activity lutein
文章编号：201917003 中图分类号： 文献标志码：
基金项目:国家自然科学基金面上项目（31772019）；天津天狮学院校级自然科学研究项目（K17003）；天津市老字号企业技术创新项目（19YFLHSN00080）
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