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投稿时间:2018-09-27
投稿时间:2018-09-27
中文摘要: 以河北石家庄玉桥传统发酵宫面面团为研究对象,利用16S rDNA 测序和18S rDNA 测序技术研究面团中微生物菌群的多样性,测序结果显示,宫面面团样品中细菌主要有:魏斯氏菌(Weissella)、发酵乳杆菌(Lactobacillus fermentum)、乳酸乳球菌(Lactococcus lactis)、棉籽糖乳球菌(Lactococcus raffinolactis)、嗜热链球菌(Streptococcus thermophilus)和一些不能培养的细菌(Uncultured Bacterium);面团中含有的酵母菌主要有:热带假丝酵母(Candida tropicalis)、异常威克汉姆酵母(Wickerhamomyces anomalus)、东方伊萨酵母(Issatchenkia orientalis)和一些不能培养的酵母(Uncultured saccharomycete)。另外,对宫面面团中的乳酸菌和酵母菌进行了平板划线分离鉴定,鉴定得到发酵乳杆菌(Lactobacillus fermentum)、嗜热链球菌(Streptococcus thermophilus)、乳酸乳球菌(Lactococcus lactis)、异常威克汉姆酵母(Wickerhamomyces anomalus)和东方伊萨酵母(Issatchenkia orientalis)。
Abstract:The diversity of the microbial flora in Yuqiao fermentation palace was researched by 16S rDNA and 18S rDNA sequencing technology.The results showed that the bacteria contain Weissella,Lactobacillus fermentum,Lactococcus lactis,Lactococcus raffinolactis,Streptococcus thermophilus and some can't cultivate bacteria and the yeast are consist of Candida tropicalis,Wickerhamomyces anomalus,Issatchenkia orientalis,and some uncultured yeast.In addition,Lactobacillus fermentation,Lactococcus raffinolactis,Lactococcus lactis,Wickerhamomyces anomalus and Issatchenkia orientalis were identified through the method of marking on a flat plate pasta dough.
keywords: pasta dough 16S rDNA 18S rDNA bacteria yeast
文章编号:201911036 中图分类号: 文献标志码:
基金项目:天津市科技计划项目(17KPHDSF00120)
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