Abstract:In order to obtain collagen efficiently，collagen was extracted by ultrasonic-double enzyme method and double enzyme method from the fish skin of Lophiiformes，which was used as raw material.The flavor protease content，alkaline protease content，ultrasonic time，enzymatic hydrolysis time，enzymatic hydrolysis temperature and pH were taken as experimental factors.And the optimal extraction process of collagen was determined by orthogonal test.The best extraction process was compared and analyzed，and a better extraction method was obtained.The results showed that the optimum extraction conditions of collagen by ultrasonic-dual enzymatic method were as follows：flavor protease addition 3 000 U/g，alkaline protease addition 5 000 U/g，ultrasonic time 70 min，enzymatic hydrolysis time 5 h，enzymatic hydrolysis temperature 50 ℃.Under these conditions，the extraction rate of collagen was （8.86 ± 0.64）%；The optimal extraction conditions for collagen extraction by double enzyme method were：flavor proteinase addition 5 000 U/g，alkaline protease addition amount 5 000 U/g.The enzymatic hydrolysis temperature was 55 ℃ and pH 8.0.Under this condition，the extraction rate of collagen was（4.55 ± 0.20）%.The addition of flavor protease was 2 000 U/g more than that of ultrasonic-double enzyme method，and the extraction rate of collagen was 4.31%lower than that of ultrasonicdouble enzyme method.In conclusion，ultrasonic double enzyme method was used to extract collagen from fish skin of Lophiiformes.