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食品研究与开发:2019,40(10):74-79
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超声波辅助酶法提取毛木耳多糖工艺条件优化
(1.山东农业大学食品科学与工程学院,山东 泰安 271018;2.山东省农业科学院农产品研究所,山东 济南 250100;3.山东省农产品精深加工技术重点实验室,山东 济南 250100;4.农业部新食品资源加工重点实验室,山东 济南 250100)
Optimization of Ultrasonic Assisted Enzymatic Extraction of Polysaccharides from Auricularia polytricha
(1.College of Food Science and Engineering,Shandong Agricultural University,Tai'an,271018,Shandong,China;2.Institute of Agro-Food Science and Technology,Shandong Academy of Agricultural Sciences,Jinan 250100,Shandong,China;3.Key Laboratory of Agro-Products Processing Technology of Shandong Province,Jinan 250100,Shandong,China;4.Key Laboratory of Novel Food Resources Processing,Ministry of Agriculture,Jinan 250100,Shandong,China)
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投稿时间:2018-08-26    
中文摘要: 以山东省鱼台县毛木耳为原料,采用超声波辅助酶法进行毛木耳多糖的提取工艺优化。首先采用单因素试验的方法研究液料比、超声波时间、超声波功率、复合酶(复合蛋白酶与纤维素酶质量比为1∶1)、酶解温度4个单因素对毛木耳多糖提取效果的影响。在单因素试验结果的的基础上,进行响应面法试验。依据响应面试验结果分析确定最优超声波辅助酶提取多糖工艺条件为:液料比40∶1(mL/g)、超声波时间31 min、超声波功率160 W、复合酶酶解温度为64 ℃,在此条件下多糖提取率为14.41%。
Abstract:Taking the Auricularia polytricha of Yutai county,Shandong province as raw material,and optimization of extraction process of polysaccharides from Auricularia polytricha by ultrasonic assisted enzymatic method.Firstly,single factor experiment was used to study the effects of liquid-to-material ratio,ultra-sonic time,ultrasonic power,and complex enzymolysis temperature(the mass ratio of complex protease to cellulase was 1∶1)on the extraction of polysaccharides from Auricularia polytricha.Based on the results of singlefactor experiments,response surface method experiments were performed.According to the regression analysis,the optimum conditions for ultrasonic-assisted enzyme extraction of polysaccharides were determined as follows:liquid-to-material ratio 40∶1(mL/g),ultrasonic time 31 minute,ultrasonic power 160 W,and complex enzymeenzymolysistemperature64 ℃.Undertheseconditions,theextractionrateofpolysaccharidewas14.41%.
文章编号:201910014     中图分类号:    文献标志码:
基金项目:山东省自然科学基金项目(ZR2018LC018);山东省重点研发计划项目(2017GNC13106);山东省现代农业产业技术体系食用菌产业创新团队建设项目(SDAIT-07-08)
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