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投稿时间：2018-09-16
投稿时间：2018-09-16
中文摘要: 对XAD-7型大孔吸附树脂纯化黑果腺肋花楸多酚的条件进行研究。以没食子酸为标准品，采用Folin-Phenol法测定黑果腺肋花楸多酚的含量，通过静态和动态吸附-解吸试验，考察了上样液浓度和pH值、上样流速、上样量、洗脱剂体积分数和pH值、洗脱流速、洗脱体积等因素对多酚吸附率和解吸率的影响。结果表明：XAD-7型大孔树脂静态吸附黑果腺肋花楸多酚的工艺条件为：上样液质量浓度2.67 mg/mL，上样液pH值4.0，吸附时间2 h；静态解吸工艺条件为：洗脱剂乙醇体积分数95%，pH值7.0，解吸时间1 h。黑果腺肋花楸多酚的动态吸附-解吸工艺条件为：上样量430 mL，上样流速1 mL/min，洗脱体积210 mL，洗脱流速1 mL/min。通过动态吸附-解吸后，黑果腺肋花楸多酚粗提物的纯度由9.56%提高到74.26%，表明XAD-7型大孔树脂法是纯化黑果腺肋花楸多酚的有效方法。
Abstract:The purification condition of polyphenol derived from chokeberry（Aronia melanocarpa）berries by macroporous resin XAD-7 was studied. Keeping gallic acid as the standard，the content of polyphenols in the chokeberry was determined by Folin-Phenol method.The static and dynamic adsorption-desorption experiments were carried out to investigate the effects of concentration and pH value of the sample，loading flow rate，sample volume，the volume fraction and pH value of the eluent，elution flow rate，elution volume and other factors on the adsorption rate and desorption rate. The results showed that the static adsorption conditions of polyphenols were that the mass concentration of the sample solution was 2.67 mg/mL，pH value of the sample solution was 4.0，the adsorption time was 2 h；the desorption process conditions were that the ethanol concentration was 95 %，the pH value was 7.0，and the desorption time was 1 h.The conditions of dynamic adsorption-desorption process were that the sample volume was 430 mL，the loading flow rate was 1 mL/min，the elution volume was 210 mL，and the elution flow rate was 1 mL/min. Under the conditions，the purity of polyphenols extractive increased from 9.56 % to 74.26 %，which indicated that macroporous resin XAD-7 could effectively purify polyphenols.
文章编号：201908027 中图分类号： 文献标志码：
基金项目:辽宁省自然科学基金项目（201801999）；沈阳市科技计划项目（F2016-205-1-22）
| Author Name | Affiliation |
| ZHANG Hui，WANG Ya-ru，LIU Yang-feng | Pharmaceutical & Bioengineering College，Shenyang University of Chemical Technology，Shenyang 110142，Liaoning，China |
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