Abstract:With dried Eleocharis tuberosa peel as raw materials，pigment was extracted from Eleocharis tuberosa peel by papain，the extraction effect was reflected with absorbency，and the extraction technological conditions were optimized with single-factors experiments and orthogonal tests. The result showed that the optimal extraction conditions were ethanol concentration of 90%，enzymatic dosage of 0.7%，liquid-solid ratio of 35∶1（mL/g），extraction temperature of 60 ℃，and extraction time of 5 h.Under the optimal conditions，the absorbency of the extract，which was constant-volumed in 50 mL volumetric flask and then was diluted five times，could reach 0.896，which was largely higher than the absorbency of 0.769 without enzyme at the same conditions.