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投稿时间：2018-08-20
投稿时间：2018-08-20
中文摘要: 建立蛴螬多肽的制备工艺，探究多肽的抗氧化活性。采用酶法将蛴螬制备成蛴螬蛋白水解肽，以蛴螬多肽提取率及其总抗氧化能力为衡量指标，通过单因素试验确定了酶种类、酶虫比、底物浓度、水解体系pH值、酶解温度和时间对蛴螬多肽提取率及其总抗氧化能力的影响。通过正交试验优化蛴螬多肽提取率及其总抗氧化能力，获得最佳酶解工艺，即底物浓度5%、水解体系pH 7、酶解温度40 ℃。在此试验条件下，蛴螬多肽的提取率为14.21%，总抗氧化能力为0.752 mmol/L。
Abstract:The enzymatic hydrolysis parameters of antioxidant peptide extracted from Protaetia brevitarsis larvae were optimized. The Protaetia brevitarsis larvae was prepared into proteolytic peptides by enzymolysis technology，the extration rate of Protaetia brevitarsis larvae peptides and total antioxidant capacity were chosen as the index. The effects of enzyme species，enzyme/worm ratio，substrate the concentrarion，pH value，enzymatic hydrolysis temperature and time on the extraction rate of peptides and total antioxidant capacity were determined by single-factor test. Then the conditions for the maximum extraction rate of Protaetia brevitarsislarvae peptides and total antioxidant activity were optimized by orthogonal experiments. The results indicated that the best condition for the enzymatic hydrolysis process were as follows：substrate concentration of 5%，the initial pH at 7，enzymolysis temperature of 40 ℃. Under these conditions，the extraction rate of Protaetia brevitarsis larvae peptides and and total antioxidant capacity were 14.21%and 0.752 mmol/L，respectively.
keywords: Protaetia brevitarsis larvae antioxidant peptide enzymatic hydrolysis orthogonal test preparation
文章编号：201908026 中图分类号： 文献标志码：
基金项目:
| 作者 | 单位 |
| 张海英，李秀梅，潘方方，杨培龙，俞晓亭，石冬冬，马文建 | 1.天津科技大学生物工程学院，工业发酵微生物教育部重点实验室，天津市工业微生物重点实验室，天津300457；2.中国农业科学院饲料研究所，农业部饲料生物技术重点实验室，北京100181 |
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