Abstract:To optimize the extraction process of Lycium ruthenicum protein （LRP）by response surface methodology.The anti-aging activity of LRP was studied.On the basis of single-factor test，extraction time，extraction times and liquid-to-material ratio were selected as investigation factors，the extraction process was optimized through the response surface methodology.The optimal extraction conditions were as follows：pH 7，extraction time 21h，extraction times 2 times，liquid to material ratio 17 ∶1 （mL/g）；the average protein extraction rate was 8.62%after validation test，which was close to the model predictive value.The optimized extraction process was stable and feasible.The D-galactose aging model mice were used as the research object to evaluate the anti-aging effect of LRP on aging model mice.Kunming mice were randomly divided into a blank control group，an aging model group，and low-dose，medium-dose，and high-dose group of LRP content（125，250，500 mg/kg，respectively）.After 30 days，The activities of superoxide dismutase （SOD）and glutathione peroxidase（GSH-Px），and the contents of malondialdehyde（MDA）and Catalase（CAT）in the serum were assayed.LRP could significantly reduce MDA content and increase SOD，GSH-Px and CAT activity.The results indicated that LRP had a delayed anti-aging effect on D-galactose-induced aging mice，and its mechanism might be related to the elimination of free radicals in vivo.