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投稿时间:2018-08-27
投稿时间:2018-08-27
中文摘要: 以“雪花”梨为原料,分别采用全果(剔除种子)、皮肉和果肉榨取果汁接种市售酵母RC212后酿制梨酒。对梨汁和梨酒中所含的酚类物质分别采用高效液相色谱法(high performance liquid chromatograph,HPLC)进行分析。结果表明:果汁酿制成梨酒后,总酚含量下降,其中熊果苷、没食子酸、原儿茶酸、绿原酸和芦丁含量在不同梨酒中均呈下降趋势,其中绿原酸减少幅度最大;3,4-二羟基苯丙氨酸、儿茶素含量则显著增加;槲皮素增幅较小,而表儿茶素含量则在3种梨酒中呈现不同的变化趋势。熊果苷、儿茶素和3,4-二羟基苯丙氨酸是3种梨酒中含量最高的3种酚,其余酚类物质含量均较少,不足5 μg/mL,咖啡酸含量甚至不足0.5 μg/mL。DPPH自由基清除率与酚类物质含量呈正相关,但偏最小二乘分析(partial least squares,PLS)结果显示,任何一种酚类物质对DPPH自由基清除率均没有显著影响,说明清除DPPH自由基可能是各种酚类物质协同作用的结果。
Abstract:Juice was squeezed from whole fruit(after removing seeds),peel and flesh,flesh from ′Xuehua′pear seperately,and then was inoculated with yeast strain RC212 to brew pear wine.Phenols in pear juice and wine were anlysized by high performance liquid chromatography (HPLC).Results showed that the total content of phenols decreased after juice brewed into wine,the content of arbutin,gallic acid,protocatechuic acid,chlorogenic acid and rutin declined in different pear wines,and the decrease of chlorogenic acid was the most among them;whereas the content of 3,4-dihydroxyphenylalanine and catechinic acid was both significantly inreased.The content of quercetin was slightly rising,and epicatechin content showed different changing tendency in these three pear wines.The contents of Arbutin,catechinic acid and 3,4-dihydroxyphenylalanine were the highest in the three pear wines,while the content of the other phenols was no more than 5 μg/mL each,the content of caffeic acid was even less than 0.5 μg/mL.DPPH radical scavening ability was positively correlated with the total content of phenols.But the result of partial least squares(PLS)analysis revealed none of phenols had significantly influence on the DPPH radical scavening ability,showing DPPH radical scavening ability might be the consequence of synergistic effect of different phenols.
文章编号:201907013 中图分类号: 文献标志码:
基金项目:国家梨产业技术体系(CARS-28-22);河北省现代农业科技创新工程(494-0402-YBN-XNS4)
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