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食品研究与开发:2019,40(7):79-83
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武夷岩茶粗老叶酶法提取高含量游离氨基酸水提液的工艺研究
(1.武夷学院茶与食品学院,福建武夷山354300;2.江南大学食品科学与技术国家重点实验室,江苏无锡214122)
Study on the Technology of Extracting High Free Amino Acid Water Extract by Enzymatic Immersion of Crude Old Leaves of Wuyi Rock Tea
(1.School of Tea and Food Science,Wuyi University,Wuyishan 354300,Fujian,China;2.State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,Jiangsu,China)
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投稿时间:2018-10-14    
中文摘要: 针对武夷岩茶制作过程中产生大量下脚料如粗老叶、茶梗等问题,以粗老叶为原料,添加木瓜蛋白酶和纤维素酶辅助浸提,以提高水提液的营养及风味,为下一步制备茶饮料及速溶茶粉提供原料和理论依据。以游离氨基酸总量为指标,选取两种酶复配比、总加酶量、浸提温度、浸提时间和料液比进行单因素试验,再通过正交试验优化工艺参数。结果表明:最佳浸提工艺参数为纤维素酶与木瓜蛋白酶以酶活力比(U/U)5∶5复配、总加酶量1 000 U、酶解温度50℃、酶解时间25 min、料液比1∶22(g/mL)。在此工艺条件下得到的水提液中游离氨基酸总量最高,可达26.97%。
中文关键词: 武夷岩茶  粗老叶  酶法  游离氨基酸  水提液
Abstract:To deal with the problem during the process of making Wuyi rock tea,a lot of leftover such as crude old leaves and tea stems would be produced,the crude old leaves were used as raw materials in this study,and the papain and cellulase were added to assist the extraction,so as to improve the nutrition and the flavor of the aqueous extract and provide the materials and basis for the next step of preparing tea beverages and instant tea powder.Taking the total amount of free amino acids as the index,the single factor experiment was carried out by combining the two enzymes,the total enzyme amount,the extraction temperature,the extraction time and the ratio of material to liquid,and then the orthogonal experiment was used to optimize the process.The results showed that the optimal extraction process was the combination of cellulase and papain with enzyme activity ratio(U/U)5∶5,total enzyme amount 1 000 U,enzymatic hydrolysis temperature 50℃,enzymatic hydrolysis time 25 min,the liquid ratio was 1 ∶22(g/mL).The total amount of free amino acids in the aqueous extract obtained under this process condition is the highest,being up to 26.97%.
文章编号:201907014     中图分类号:    文献标志码:
基金项目:国家自然科学基金(21407118);福建省高校新世纪优秀人才支持计划(闽教科[2016]23号);校科研基金平台项目(XP201801)
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