Abstract:Abstract：This study researched the optimum extraction process of flavonoids from Hedyotis diffusa and Scutellaria barbata，and the antioxidant activity of the flavonoids extraction solution under the optimum extraction conditions.Response surface test in the research was on the basis of single factor experiment，then the three factor and three level response surface methods were designed for the extraction technology of flavonoids.The results showed that the best combination of experimental factors for obtaining higher total flavonoids yield was ethanol concentration of 74%，liquid-solid ratio of 70 ∶1（mL/g），and extraction time of 5 h.Under these conditions，the total yield of flavonoids was 7.26%.The flavonoids obtained by this process had strong scavenging capacity for hydroxyl radicals and superoxide free radicals：when the concentration of flavonoids was 0.4 mg/mL，the scavenging rate of flavonoids on hydroxyl radical got the highest，which was 57.16%；when the concentration of flavonoids was 0.3 mg/mL，the scavenging rate of flavonoids on superoxide anion radical got the highest，which was 63.89%.