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食品研究与开发:2019,40(4):86-90
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复合酶提取洋葱多糖的工艺研究
(内江师范学院化学化工学院,四川内江641199)
Extraction Process of Onion Polysaccharides by Compound Enzyme
(College of Chemistry and Chemical Engineering,Neijiang Normal University,Neijiang 641199,Sichuan,China)
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投稿时间:2018-08-22    
中文摘要: 以洋葱为原料,采用复合酶(纤维素酶、木瓜蛋白酶和中性蛋白酶)酶解法提取洋葱多糖。考察复合酶用量、时间、温度、pH值、料液比等因素对洋葱多糖的纯度和收率的影响。通过单因素试验与正交试验优化复合酶提取洋葱多糖的工艺。结果表明:复合酶用量为0.3%,酶解温度为50℃,酶解时间为75 min,缓冲溶液pH值为6.0,料液比为1∶35(g/mL)时,多糖收率为18.75%,纯度达62.00%。
中文关键词: 洋葱  多糖  提取  复合酶  苯酚硫酸法
Abstract:Abstract:The onion polysaccharides were extracted by compound enzyme of cellulose,papain and neutralprotease from onion. The effects of compound enzyme concentration,extraction time,extraction temperature,pHand liquid-solid ratio were investigated. The extraction technology was optimized by orthogonal experimentationon the basis of a series of single -factor experiments. The optimal conditions of extraction were obtained asfollows:compound enzyme dosage was 0.3 %,enzymolysis temperature was 50 ℃,enzymolysis time was 75min,buffer solution pH was 6.0,and solid-liquid ratio was 1 ∶ 35(g/mL),the polysaccharides yield reached18.75 % and the purity reached 62.00 %. The yield and purity of onion polysaccharide was improved bycompound enzyme.
文章编号:201904017     中图分类号:    文献标志码:
基金项目:基金项目:四川省教育厅成果转化培育项目(13CZ0002);四川省大学生创新创业训练计划项目(X201644)
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