Abstract:The present study was designed to determine the effect and mechanism of resveratrol for anti-fatigue through exhaustive treadmill exercise model.The rats were divided into 4 groups，namely the control（C），lowdose resveratrol，medium-dose resveratrol and high-dose resveratrol groups，which received distilled water and various doses of resveratrol for 4 weeks.After 4 weeks，the rats were subjected to the exhaustive treadmill exercise；the exhaustive time and fatigue-related biochemical parameters，including blood lactic acid（BLA），blood urea nitrogen （BUN），liver glycogen，muscle glycogen，and superoxide dismutase（SOD），glutathione peroxidase（GPx），catalase（CAT），malondialdehyde（MDA）in liver，were measured.The result showed that resveratrol had an anti-fatigue effect，which prolonged exhaustive time，decreasing the levels of BLA，BUN and MDA in liver，increasing the levels of liver glycogen，muscle glycogen，and SOD，GPx，CAT in liver.The anti-fatigue mechanisms of resveratrol might be attributable to various factors：1）resveratrol could reduce the accumulation of metabolites or rapidly remove metabolites；2）resveratrol could improve exercise metabolism control and energy metabolism activation；3）resveratrol could increase antioxidant enzyme activities in vivo，forming a synergistic antioxidant network with endogenous antioxidants to reduce oxidative stress；4）resveratrol can reduce free radical-induced lipid peroxidation and protect liver cell membrane integrity.Resveratrol may be used as a new functional food or anti-fatigue supplement in sports nutrition.