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投稿时间:2018-04-02
投稿时间:2018-04-02
中文摘要: 探究炮制对广山楂中儿茶素、柚皮苷、根皮苷3种成分含量的影响。方法采用SHIMADZU VP-ODS C18柱(250 mm×4.6 mm,5 μm),流动相:乙腈(A)-0.2%磷酸水溶液(B),梯度洗脱;流速 0.8 mL/min;紫外检测波长为 285 nm;柱温30℃。结果表明:各炮制品中儿茶素含量如下:炒焦品(14.23 mg/g)>盐炙品(12.53 mg/g)>炒黄品(7.35 mg/g)>炒炭品(7.23 mg/g)>酒炙品(5.12 mg/g)>生品(3.71 mg/g);柚皮苷含量为:酒炙品(6.18 mg/g)>炒焦品(5.35 mg/g)>盐炙品(4.66 mg/g)>炒黄品(3.26 mg/g)>生品(2.54 mg/g)>炒炭品(1.87 mg/g)。根皮苷含量依次是:盐炙品(0.98 mg/g)>炒焦品(0.82 mg/g)>酒炙品(0.76 mg/g)>炒黄品(0.43 mg/g)>生品(0.37 mg/g)>炒炭品(0.27 mg/g)。不同炮制方法对广山楂中成分产生一定影响,盐炙等炮制方法可提高广山楂中儿茶素、柚皮苷、根皮苷含量,炒炭后则使柚皮苷、根皮苷含量降低。
Abstract:To explore the effect on the three contents of catechin,naringin and phlorizin in Malusdoumeri(Bois.)Chev by different processing methods.A SHIMADZU VP-ODS C18(250 mm × 4.6 mm,5 μm)column was used.The separation was achieved using acetonitrile(phase B)and 0.2%phosphoric acid in water(phase A)with a linear gradient,the flow rate was 0.8 mL/min,the detection wavelengths was set at 285 nm,the column temperature was set at 30℃.The results showed that:The content of catechin in different processed products of Malusdoumeri(Bois.)Chev were as folllows:parched sample(14.23 mg/g)> salt prepared(12.53 mg/g)>yellow-parched sample(7.35 mg/g)>carbonized sample (7.23 mg/g)> wine prepared(5.12 mg/g)> raw materials(3.71 mg/g).The contents of naringin were as follows:wine prepared(6.18 mg/g)> parched sample(5.35 mg/g)> salt prepared(4.66 mg/g)> yellow-parched sample(3.26 mg/g)> raw materials(2.54 mg/g)>carbonized sample(1.87 mg/g).The contents of phlorizin were as follows:salt prepared(0.98 mg/g)>parched sample(0.82 mg/g)> wine prepared(0.76 mg/g)> yellow-parched sample(0.43 mg/g)> raw materials(0.37 mg/g)>carbonized sample (0.27 mg/g).Different processing methods might have some affection on the quality of Malusdoumeri(Bois.)Chev.Processed,and the methods of salt prepared and so on increased the contents of catechin,naringin and phlorizin,while the processing method of carbonized reduced the contents of naringin and phlorizin in Malusdoumeri(Bois.)Chev.
keywords: Malusdoumeri(Bois.)Chev processing methods catechin naringin phlorizin high performance liquid chromatography(HPLC)
文章编号:201824022 中图分类号: 文献标志码:
基金项目:广西中医药大学中药学优势学科建设专项资助课题(050170035);广西中药药效研究重点实验室课题(17-259-20);广西中医药大学2017年校级科研项目(2017MS018);广西中医药大学2017年校级大学生科研项目(DXS17011);广西中医药大学2017大学生创新创业训练计划项目(201710600141);广西中医药大学2017大学生创新创业训练计划项目(201710600150)
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