Abstract:This experiment with Tricholoma Lobayense fruiting body used different methods respectively to detect the basic composition and the content of flavor substance.The crude fat，crude protein，ash content，soluble sugar soluble protein content，free amino acid and 5'-nucleotide content were determined by physical and chemical analysis methods，and then the equivalent umami concentration（EUC）was calculated.The crude protein content was 17.34 g/100 g，crude fat content was 5.86 g/100 g，ash content was 7.03 g/100 g，soluble sugar content was 30.32 g/100 g（dry weight），and soluble protein content was 2.84 g/100 g（dry weight），total phenol content of 148.96 GAE mg/g（dry weight）.The total free amino acid was 17.07 mg/g.All kinds of flavor nucleotide content was 2.22 mg/g.According to the content of free amino acid and 5'-nucleotide，EUC was 31.65 g/100 g.By analyzing the basic components we know that crude protein content was high and fat content was low ，meanwhile fruiting body contains soluble protein，soluble sugar，and a certain amount of total phenol.By analyzing the free amino acid and nucleotide，ECU belonged to the third level.