Abstract:A high performance liauid chromatograhy method was established for the determination of 11 kinds of fluorescent whitening agent in rice.The rice was diluted by 40%acetonitrile-water（adjusted pH to 11.5 by triethylamine），concentrated with nitrogen，separated by C18 column，and determined by photo-diode array at 350 nm wavelength with external standard method for quantification.The limits of the detection（LOD）of the 11 kinds of fluorescent whitening agent which were detected by high performance liquid chromatography in rice were all 0.1 μg/g.The recoveries of the 11 kinds of fluorescent whitening agent were all between59.3%-89.0%when spiked at 0.5，0.8 and 1.0 μg/mL.The relative standard deviations（RSD）of the method were 1.1%-5.8%（n=6）.