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投稿时间：2018-07-27
投稿时间：2018-07-27
中文摘要: 建立高效液相色谱-蒸发光散射检测器法（high performance liquid chromatography-evaporative light scattering detector，HPLC-ELSD）同时测定保健食品银杏叶刺梨胶囊中银杏萜类内酯含量的方法。色谱柱为Waters Symmetry C18色谱柱（250 mm ×4.6 mm，5 μm），柱温为 35℃；流动相为甲醇-水 =30 ∶70（体积比），流速 1.0 mL/min；ELSD 检测器雾化温度100℃，雾化气为空气，气流速度2.5 L/min。白果内酯、银杏内酯A、银杏内酯B、银杏内酯C分别在4.376 μg～3.128 μg，4.176 μg～12.528 μg，4.320 μg～12.960 μg，4.368 μg～13.104 μg 之间线性关系良好，平均加样回收率分别为100.86%、101.52%、99.60%、100.69%。该方法可准确测定银杏叶刺梨胶囊中银杏萜类内酯的含量，可用于该产品的质量分析。
Abstract:A HPLC-ELSD method was developed to determine the content of Ginkgo Terpene Lactones in health food ginkgo leaf and Rosa roxburghii capsule.The HPLC was performed on Waters Symmetry C18（250 mm ×4.6 mm，5 μm）column，mobile phase consisted methanol-H2O （30∶70，volume ratio），column temperature was set at 35℃，and flow rate was 1.0 mL/min.The temperature of drift tube was 100℃and the flow rate of gas was 2.5 L/min.A good linearity was obtained in the range of 4.376 μg-13.128 μg of bilobalide，4.176 μg-12.528 μg of ginkgolide A，4.320 μg-12.960 μg of ginkgolide B，4.368 μg-13.104 μg of ginkgolide C.The average recovery rates of bilobalide，ginkgolide A，ginkgolide B，and ginkgolide C were 100.86%，101.52%，99.60%，and 100.69%，respectively.The established method was suitable for quality control of ginkgo leaf and Rosa roxburghii capsule.
keywords: high performance liquid chromatography ginkgo leaf and Rosa roxburghii capsule ginkgolides determination of content quality analysis
文章编号：201823028 中图分类号： 文献标志码：
基金项目:
| Author Name | Affiliation |
| ZHOU Ya-jie，FENG Peng*，GUO Yuan，FENG Min | （Nanjing Zhongke Pharmaceutical Co.，Ltd.，Nanjing 210008，Jiangsu，China） |
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