Abstract:Abstract：A high performance liquid chromatography（HPLC）method was established for the determination of linalool in peanut stems and leaves at different stages.Based on the single factor experiments，orthogonal experiment was used to optimize extraction conditions.The best HPLC conditions were as follows：the analytical column was a Venusil XBP C18；the mobile phase consisted of acetonitrile-water （1 ∶1，volume ratio）；flow rate was 1.0 mL/min，and detection wavelength was 205 nm.In the range of 0.2 μg-1.0 μg，there was a good linear relationship between linalool concentration and the peak area.The average recovery of the method was 100.25%with a relative standard deviation（RSD）of 1.40%.The optimal extraction conditions for linalool in peanut leaves were：ratio of material to liquid 1∶100 （g/mL），extraction temperature 90℃，extraction time 20 min.The contents of linalool in peanut leaves were higher than that in stems at all stages.At initial flowering stage，the content of linalool in peanut leaves was 4.0 times that of stems.At the ripening stage，the linalool content was 3.5 times that of stems.