Abstract:Abstract：In order to explore the comprehensive utilization approach of crude glycerol，the high-producing strain of using crude glycerol fermentation for producing lipid was selected in 4 Lipomyces yeast strain by plate culture and shake-flask fermentation，the effect of carbon and nitrogen source in culture medium on fermenting crude glycerol for producing lipid of the selected strain was studied.On the basis，fermenting course of the selected strain was studied，and the fatty acids of lipids were analysed.The results showed that Lipomyces starkeyi ZW-25 was good at fermenting crude glycerol for producing lipid，the suitable carbon and nitrogen source composition in culture medium was such as follows：crude glycerol 40 g/L，yeast powder 0.5 g/L，（NH4）2SO42.0 g/L.The biomass，lipid yield，lipid content and lipid coefficient could reach 13.79 g/L，6.97 g/L，50.52%and 18.12 under these conditions.The suitable time of fermentation for producing lipid by Lipomyces starkeyi ZW-25 was 144 h，and the main fatty acids of lipids were C16and C18fatty acids.These conclusions showed that Lipomyces starkeyi ZW-25 has good application potential，it also provided a new approach for the comprehensive utilization of crude glycerol.