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食品研究与开发:2018,39(21):167-171
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黄曲霉毒素B1降解菌株的筛选及鉴定研究
于丽娜,王明清,张初署,徐念均,赵玉华,毕洁,孙杰,龚魁杰,刘开昌,杨庆利
((1.山东省花生研究所,山东青岛266100;2.西海岸现代农业示范区管委会,山东青岛266000;3.胶南市种子公司,山东青岛266000;4.山东省农业科学院作物研究所,山东济南250000;5.青岛农业大学,山东青岛266109))
Screening and Identification of Aflatoxin B1Degradation Strains
YU Li-na,WANG Ming-qing,ZHANG Chu-shu,XU Nian-jun,ZHAO Yu-hua,BI Jie,SUN Jie,GONG Kui-jie,LIU Kai-chang,YANG Qing-li
((1.Shandong Peanut Research Institute,Qingdao 266100,Shandong,China;2.Management Committee of West Coast Modern Agricultural Demonstration Area,Qingdao 266000,Shandong,China;3.Jiaonan Seed Company,Qingdao 266000,Shandong,China;4.Crop Research Institute,Shandong Academy of Agricultural Sciences,Jinan 250000,Shandong,China;5.Qingdao Agricultural University,Qingdao 266109,Shandong,China))
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投稿时间:2018-05-15    
中文摘要: 黄曲霉毒素B1(aflatoxin B1,AFB1)污染花生、玉米等农产品和食品,具有极强的毒性和致癌性。采用香豆素为唯一碳源从海水中分离降解AFB1菌株,其中菌株M8能降解71.8%的AFB1。通过上清液、菌悬液、胞内液降解AFB1分析,初步判断菌株M8分泌至胞外的活性物质主导AFB1的生物降解作用。通过菌株形态特征、生理生化特征和16S rRNA基因系统发育学分析,鉴定菌株M8属于假单胞菌属,命名为Pseudomonas sp.M8。从海水中分离菌株M8丰富了降解AFB1降解微生物资源,为研究其作用机理和应用于黄曲霉毒素的脱毒奠定了基础。
中文关键词: 黄曲霉毒素B1  降解  假单胞菌  鉴定  筛选
Abstract:Abstract:Aflatoxin B1(AFB1)contaminates peanut,corn and other agricultural products and food,and is highly toxic and carcinogenic.At present,biological detoxification methods have become a research hotspot in the degradation of AFB1.This study used coumarin as the only carbon source to isolate and degrade AFB1strains from seawater,among which the strain M8 could degrade 71.8%of AFB1.The results showed that degradation activity of strain M8 was located in extracellular fluid.Through morphological,physiological and biochemical analysis and the evolution of 16S rRNA system,the bacterial strain M8 was identified as Pseudomonas sp.M8.The isolation of strain M8 from the seawater enriched the microbial resources and laid a foundation for the study of its degradation mechanism and the detoxification of aflatoxin.
文章编号:201821028     中图分类号:    文献标志码:
基金项目:山东省自然科学基金(ZR2016CM43、ZR2017MC060、ZR2016YL021、ZR2017MC062);山东省农业科学院重大科技成果培育计划(2016CGPY10);山东省农业科学院农业科技创新工程(CXGC2016B17、CXGC2018E21);山东省2018年度农业重大应用技术创新项目;国家重点研发计划(2018YFD0300605)
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