Abstract:The anti-aging effects of Osmanthus extract on anti-oxidation and stress in male drosophila melanogaster was studied.Osmanthus Fragrans was extracted with ethanol and concentrated under reduced pressure to obtain ethanol extract of Osmanthus Fragrans.Two-day-old male fruit fly adults were collected and randomly divided into control group and Osmanthus extract dose group （2.5，5，10，15 mg/mL），A total of 5 groups were used to test the survival and stress of male drosophila.Mean life spans，survival curves，and in vivo protein，superoxide dismutase （SOD），catalase （CAT），malondialdehyde （MDA）and other antioxidant enzyme activity were measured.Osmanthus extract doses of 10 mg/mL and 15 mg/mL could significantly prolong the lifespan of male fruit flies （P＜0.01）；15 mg/mL could significantly increase the TP content of drosophila melanogaster in 45 d （P＜0.05）；The activities of CAT in 25 d drosophila melanogaster could be significantly increased in the 10 mg/mL and 15 mg/mL dose groups（P＜0.05），and the CAT activity in drosophila melanogaster was significantly increased in all dose groups（P＜0.05）；5 mg/mL significantly reduced the content of MDA in 25-day-old fruit fly（P＜0.05），10，15 mg/mL dose group could significantly reduce the MDA content in 25-day-old fruit fly（P＜0.01），and 10 mg/mL dose group energy significantly reduce the MDA content in drosophila melanogaster at 45 days（P＜0.05），15 mg/mL dose group could significantly reduce the MDA content in 45 d drosophila melanogaster（P＜0.01）；5，10，15 mg/mL dose group significantly increased SOD activity in 25 d and 45 d drosophila（P＜0.01）.Osmanthus extract could increase the activity of SOD，CAT and other antioxidant kinases，which could relieve the damage of free radicals caused by oxidative stress caused by body aging，and had the effect of prolonging the life span.