Abstract:The hyperglycemic mice induced by streptozotocin （STZ）were used to evaluate the auxiliary hypoglycemic function of small soluble molecule β-glucan from hull-less barley.After irrigating stomach of hyperglycemic mice β-glucan （before and after enzymolysis）dissolved in water and 15 degrees of alcohol for 30 days.Blood glucose and glucose tolerance levels were determined.The results showed that the samples of βglucan（before and after enzymolysis）from hull-less barley had no significant difference between sample group and control group on an empty stomach.There were significant differences between the sample group（dissoved in water）and model group on the levels of blood glucose and glucose area under curve （p≤0.05）while the sample dissoved in 15 degrees of alcohol don't have this effect.There was no difference between dosages as well.According to“Technical specifications for inspection and evaluation of health food”，small soluble molecule βglucan had auxiliary hypoglycemic function on mice after 30 d feeding in a row，which could be used in functional drinks.