Abstract:Peanut is an important source of food allergen and contains many sensitizing proteins，of which Ara h1 and Ara h2 are the most allergenic.This greatly influences the living quality and even threatens the life safety of people who are peanut allergy，and therefore，it's essential to evaluate how to reduce the allergenicity of peanut.Single factor experiment and orthogonal experiment designation were used，and double-antibody sandwich enyme-linked immunosorbent assay （ELISA）was used to evaluate allergen-alleviation effects，results showed that the optimal condition（alcalase∶neutrase∶papain ∶flavorzyme=2 ∶2 ∶1 ∶1，enzyme dosage was 4%，the temperature 60℃，pH 7.0 and reaction time 4 h）for reducing peanut allergen by complex enzymic reaciton was obtained；the mean allergen-alleviation ratio of Ara h1and Ara h2 in peanut pulp was 92.91%，under this condition.