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投稿时间：2017-09-08
投稿时间：2017-09-08
中文摘要: 为准确、快速地进行食品中狐狸、驴、马成分的定量检测，选择适宜高效的聚合酶，本研究利用荧光定量聚合酶链式反应（real-time Fluorescent Quantitative Polymerase Chain Reaction，FQ-PCR）的方法构建狐狸、驴、马源性成分检测的3种阳性质粒分子，对3种质粒分子的方法特异性、灵敏度进行验证，并对实时荧光PCR反应体系中脱氧核糖核酸（Deoxyribose Nucleic Acid，DNA）聚合酶效果进行对比分析。研究结果发现，所选择的3种品牌的反应液和脱氧核糖核苷三磷酸（Deoxyribonucleoside Triphosphate，dNTP）均具有很高的灵敏度，检出限略微有差异，均可满足日常检验工作的需要。
Abstract:In order to carry out quantitative detection of fox，donkey and horse in food accurately and quickly，and choose the polymerase properly and efficiently，in this study，with the method of real-time Fluorescent Quantitative Polymerase Chain Reaction（FQ-PCR），three kinds of positive plasmids（fox，donkey and horse derived components）were constructed，the specificity and sensitivity were verified，and the effectiveness of DNA polymerase in the real-time fluorescence PCR reaction system were compared and analyzed.The results showed that the PCR reaction buffer and dNTP from three common brands were high sensitive.Although the detection limits were slightly different，they still could meet the needs of daily inspection work.
文章编号：201806024 中图分类号： 文献标志码：
基金项目:山东省食品药品检验研究院项目（2016ZNKT0007）
| Author Name | Affiliation |
| GUO Ying-hui，ZHENG Shi-chao，HUO Sheng-nan* | Institute for Food and Drug Control，Jinan 250101，Shandong，China |
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