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投稿时间：2017-05-17
投稿时间：2017-05-17
中文摘要: 以红花籽粕为原料，探讨胰蛋白酶（parenzyme）、木瓜蛋白酶（papain）、枯草芽孢杆菌中性蛋白酶（bacillus subtilis neutral protease）3种商业酶水解红花籽粕中的氨基酸态氮并研究其最佳水解工艺条件。结果表明：胰蛋白酶parenzyme水解红花籽粕的正交试验优化条件为料液比1∶3（g/mL）、酶用量为2%、酶解温度为45℃、酶作用时间3.0 h，此条件下水解红花籽粕中的氨基酸态氮含量达7.7%，木瓜蛋白酶papain水解红花籽粕的正交试验优化条件为料液比1∶3（g/mL）、酶用量为2.0%、酶解温度为55℃、酶作用时间4.0 h，此条件下水解红花籽粕中的氨基酸态氮含量达8.2%，枯草芽孢杆菌中性蛋白酶bacillus subtilis neutral protease水解红花籽粕的正交试验优化条件为料液比1∶3（g/mL）、酶用量为2.0%、酶解温度为40℃、酶作用时间4.0 h，此条件下水解红花籽粕中的氨基酸态氮含量达4.27%。
中文关键词: 红花籽粕 水解 胰蛋白酶 枯草芽孢杆菌中性蛋白酶 木瓜蛋白酶
Abstract:The effects of three commercial enzymes，parenzyme，papain and bacillus subtilis neutral protease，on the hydrolysis of amino acid nitrogen in safflower meal and the optimum hydrolysis conditions were studied.The results showed that the optimum conditions for the parenzyme hydrolysis were as follows：the ratio of solid to liquid 1 ∶3（g/mL），enzyme dosage2%，hydrolysis temperature 45 ℃，enzymatic hydrolysis time 3.0 h.Under the optimum conditions，the content of amino acid nitrogen in the hydrolyzed safflower meal reached 7.7%.The optimum conditions for the papain hydrolysis were as follows：the ratio of solid to liquid 1 ∶3（g/mL），enzyme dosage 2.0%，hydrolysis temperature 55℃，enzymatic hydrolysis time 4.0 h.Under this condition，the content of amino acid nitrogen in the hydrolyzed safflower meal reached 8.2%.The optimum conditions for the bacillus subtilis neutral protease hydrolysis were as follows：the ratio of material to liquid 1 ∶3（g/mL），enzyme dosage 2.0%，hydrolysis temperature 40℃，enzymatic hydrolysis time 4.0 h.Under this condition，the content of amino acid nitrogen in the hydrolyzed safflower seed meal reached 4.27%.
文章编号：201804021 中图分类号： 文献标志码：
基金项目:武汉工商学院校级科研项目（A2016014）
| Author Name | Affiliation |
| WEI Na，LÜ Kai-bo* | ExperimentalTeaching Center ofWuhanTechnology andBusiness University，Wuhan430065，Hubei，China |
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