Abstract:The purpose was to establish Immunomagnetic Separation and PCR-Lateral flow dipstick method for identification of bovine ingredient in food.Immunomagnetic separate DNA of food，and build the PCR-Lateral flow dipstick method through the design of specific primers and detect the specificity，sensitivity and stability of the method.The detection sensitivity was 0.01%.The target fragment with length as 561 bp was amplified from bovine sample，indicating that the primers had good specificity.PCR-Lateral flow dipstick method combined with immunomagnetic separation had strong specificity，high sensitivity，simple，and rapid，which was an effective molecular biological detection technique for identification of bovine ingredient in food.