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投稿时间:2017-07-11
投稿时间:2017-07-11
中文摘要: 研究红曲固态发酵过程中总糖、还原糖、多糖和总淀粉含量的变化规律,以期为其炮制机理及药效物质基础提供参考。采用四川得恩德药业有限公司不同发酵天数红曲,利用酶解法水解淀粉,盐酸水解提取总糖,高温水提取还原糖和多糖。以无水葡萄糖为对照品,分别采用3,5-二硝基水杨酸法(DNS法)测定总糖和还原糖含量,同时折算淀粉含量;蒽酮-硫酸法检测多糖含量,同时进行方法学考察。结果表明:红曲淀粉含量由初期60.78%下降至后期19.78%;红曲总糖含量由发酵初始的76.77%下降至45%;还原糖含量在发酵9 d以后趋于稳定为1.2%左右;多糖在30 d时达到最大值6.14%。随着发酵天数的变化,红曲中糖类物质有一定的变化规律,可为炮制机理及药效基础提供一定参考依据。
Abstract:To research the variation of both total sugar,polysaccharide ,reducing sugar and total starch in solid ferment monascus during fermentation ,and providing reference for its processing mechanism and pharmacodynamic material foundation.Solid ferment monascus product were provide by Sichuan De En De Pharmaceutical Limited Company.The starch was decomposed by enzymatic hydrolysis.Total sugar of solid fermentation of monascus was extractied by hydrochloric acid hydrolysis method,reducing sugar and polysaccharide were extractied by high temperature water.With glucose as reference,the content of total sugar ,reducing sugar and starch were determined by 3,5-dinitrosalicylic acid(DNS)method,and the content of polysaccharide was determined by anthrone-sulfuric acid method.At the same time,the methodology was investigated.Results showed that:starch content of monascus from the initial 60.78%down to the late 19.78% ,the total sugar content of monascus decreased from 76.77%to 45%of the initial,the content of reducing sugar tended to be stable after 9 d to 1.2%,monascus polysaccharides on 30 d reached the maximum value of 6.14%,then the starch content followed by a downward trend.With the change of the duration of the fermentation of monascus,carbohydrate changes disciplinary,It can provide some references for its processing mechanism and pharmacodynamic material foundation.
keywords: solid ferment monascus product total sugar reducing sugar polysaccharide starch content determination
文章编号:201803015 中图分类号: 文献标志码:
基金项目:国家中医药管理局公益性行业科研专项(201507004);四川省科技厅苗子工程项目(2016RZ0037)
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