Abstract:The effects of different extraction conditions on the yield of Seed of Hibiscus manihot L.polysaccharide were studied by water extraction method.In this study，the extraction rate of polysaccharide from seed of Hibiscus manihot L.was studied by using response surface methodology on the basis of single factor experiments with extraction temperature，extraction time and ratio of liquid to material.The results showed that the optimum extraction conditions were as follows：extraction temperature 71℃，extraction time 3.30 h，ratio of liquid to material 31 ∶1（mL/g）.The yield of polysaccharide was 13.58%，and the relative error with the expected value was 3.74%.The results showed that it was reasonable to use the response surface method to optimize the extraction of polysaccharide from Hibiscus manihot L..