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食品研究与开发:2018,39(1):20-24
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青刺果总黄酮超声波辅助提取及其抗氧化活性
刘招娣,刘祥义,张水滔,林早琴,李云仙,徐娟*
(西南林业大学化学工程学院,云南昆明 650224)
Ultrasonic-assisted Extraction and Antioxidant Activity of Total Flavoniods from Prinsepia utilis Royle
LIU Zhao-di,LIU Xiang-yi,ZHANG Shui-tao,LIN Zao-qin,LI Yun-xian,XU Juan*
(School of Chemical Engineering,Southwest Forestry University,Kunming 650224,Yunnan,China)
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投稿时间:2017-04-16    
中文摘要: 以青刺果油粕为原料,利用超声波辅助提取总黄酮,通过单因素试验探究各因素对青刺果总黄酮得率的影响及正交试验确定最佳工艺参数。并以VC和BHT为对照,对其还原性及羟自由基·OH和超氧阴离子自由基O2-·的清除能力进行测试。结果表明:最佳提取工艺条件为乙醇浓度60%,提取温度60℃,提取时间35min,料液比1∶30(g/mL),此条件下总黄酮的得率为5.28%。按其工艺条件制备青刺果黄酮具有较强的体外抗氧化活性,其抗氧化能力低于VC,与BHT相当。
Abstract:Ultrasonic-assisted extraction process for total flavonoids from Prinsepia utilis Royle oil meal was studied.The influence of flavonoids extraction was discussed by single factor;confirm its suitable extraction condition of flavonoids by orthogonal experiment.The evaluation of antioxidant activity was carried out by reduc-ing power,hydroxyl,superoxide anion scavenging assays in comparison to VCand BHT.The results showed that ethanol concentration 60%,extraction temperature 60℃,extraction time 35min,material-liquid radio 1 ∶30(g/mL),Under this conditions,the yield of total flavonoids from Prinsepia utilis Royle oil meal was 5.28%.Flavonoids from Prinsepia utilis Royle by ultrasonic-assisted extraction technological parameters presented strong antioxidant activity and its antioxidant capacity was equivalent to butylated hydroxyl toluene(BHT),than that of BHT,but lower than that of VC.
文章编号:201801005     中图分类号:    文献标志码:
基金项目:云南省大学生创新创业训练计划项目(S201710677007);西南林业大学科技创新项目(C17009)
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