Abstract:A method for the content determination of oleanolic acid in turnjujube was established.Ultraviolet spectroscopy，which was the best method for the content determination of color reagent and stabilizer，the coloration time of water bath and reagent and stabilizer time.The results indicated that，for the oleanolic acid，ethanol solution（75%）was found to the best extraction reagent，Vanillic aldehydet（0.4 mL，50 g/L）acetic acid was used as color reagent.Perchloric acid（0.8 mL）was used as the stabilizer，all the reactants were conducted in water bath at 70℃for 25 minates.Then the absorbance of the oleanolic acid was measured under 540 nm.Finally，the content of oleanolic acid was determined to be 4.72%.This method was determined to be the optimum method.