Abstract:An accurate and sensitive method for the detection of amantadine（AM）residues in animal-derived food products was developed by combining solid-phase extraction with high performance liquid chromatography-tandem mass spectrometry（SPE-HPLC-MS/MS）.The Oasis MCX solid-phase extraction column was used for complex matrix purification and Agilent SB-C18 column（2.1 mm ×150 mm，3.5 μm）was employed for separation in liquid chromatography using a mobile phase consisting of 0.1%formic acid and acetonitrile（80∶20，volume ratio）with the flow rate 0.2 mL/min.The electrospray ionization （ESI）source in the positive mode and the multiple-reaction monitoring（MRM）mode were used for the quantitative analysis with external standard method.The results showed that the calibration curves were in good linearity for the AM ranged from 0.1 μg/L to 100 μg/L with the correlation coefficients（R2）＞ 0.999．The limits of detection（LODs）and the limits of quantification （LOQs）of this method were 1.0 μg/kg and 3.0 μg/kg respectively．The average recovery rates were from 83.6%to 94.2%with the good relative standard deviations （RSDs，RSD＜4.0%，n=3）in chosen chicken mus cle，chicken liver，egg，beef and mutton samplesat three standard addition levelsof 30.0，60.0，and 90.0 μg/kg．