Abstract:Walnut protein is a balanced protein，it's nutritional value is similar with that of animal protein.Present work employed NaOH assisted by ultrasonic to extract the protein in walnut meal，and the effect of single factor like liquid material ratio，NaOH concentration，temperature and time had been studied.Under the condition of single factor screening，the extraction process of protein in walnut meal was optimized by response surface methodology，the results showed that the optimum extraction conditions of walnut protein were as follows：the liquid-solid ratio 40∶1（mL/g），the concentration of 0.08%，extraction temperature 58℃and extraction time 110 min，the maximum extraction rate of walnut protein was 56.51%.