Abstract:To develop fluorescence spectrophotometry and liquid chromatography-fluorescence detector methods for quick determination of tracer agent Rhodamine B in processed products from waste cooking oil.The oil samples with tracer agent Rhodamine B were extracted with anhydrous ethanol，and then use fluorescence spectrophotometry and liquid chromatography-fluorescence detector methods to determine and analyze the processed extract respectively.The result showed that these two methods had a good linearity over the range of 0.5 μg/L-30 μg/L and the limit of quantification（LOQ）were 1 μg/kg.Beside that，the average contents detected at three spiked levels（10，50，100 μg/kg）were valid.Both methods can be applied to estimate adding condition of processed product from waste cooking oil in edible oil.This research based on the tracer technique in processed products from waste cooking oil for rapid detection means and methods，which can effectively prevent waste edible oil back to the table，ensure the safety of edible oil and provide new ideas and basis for regulation of waste cooking oil.