Abstract:To optimize the process of purifying total flavonoids in rose with polyamide resin.The content of total flavonoids in roses extract was determined by UV spectrophotometry with rutin content as an index.The total flavonoids in rose were enriched and purified by the optimal process parameters that screened by single factor and orthogonal test.The optimum conditions were as follows：the concentration and pH of sample solution were 4 mg/mL and 5；the ratio of diameter to height was 1 ∶7；the temperature of loading sample was room temperature；reaching adsorption saturation within over 3 h；scouring off impurity with 15%ethanol；washing with 80%ethanol（pH 5-6，8BV）.The content of total flavonoids in the dried powder was rised to 23.4%，which was about 5.7 times as much as before purification.