本文已被：浏览 1296次 下载 763次
投稿时间：2017-02-21
投稿时间：2017-02-21
中文摘要: 运用高速逆流色谱技术从铁皮石斛中分离制备出芥子酸，为石斛属内首次分离得到。分离采用的溶剂体系为石油醚-乙酸乙酯-甲醇-水（4∶6∶3∶7，体积比），上相做固定相，下相做流动相，流速2 mL/min，转速800 r/min，检测波长280 nm。单次进样石斛提取物200 mg，分离出纯度为93.11%的芥子酸20.34 mg，固定相保留率46.67%。体外抗氧化结果显示，芥子酸有较强的DPPH自由基清除能力，IC50为0.046 mg/mL。
中文关键词: 铁皮石斛 高速逆流色谱 芥子酸 1，1-二苯基-2-三硝基苯肼
Abstract:Sinapic acid was separated and purified from Derdrobium officinale by high-speed countercurrent chromatography，which was isolated for the first time from Dendrobium.Ether-ethyl acetate-methanol-water（4∶6∶3∶7，volume ratio）was used as the two-phase solvent system，in which the upper and lower phase were used as the stationary and mobile phase，respectively.The flow rate was set at 2 mL/min，and the revolution speed was 800 r/min.The detection wavelength was 280 nm and the retention of stationary phase was 46.67%.Finally，20.34 mg of sinapic acid with a purity of 93.11%was obtained in a one-step separation from 200 mg of crude extracts.The results showed that sinapic acid had a strong DPPH radical scavenging ability in vitro and the IC50was 0.046 mg/mL.
keywords: Derdrobium officinale high-speed countercurrent chromatography sinapic acid 1,1-Diphenyl-2-picrylhydrazyl
文章编号：201721005 中图分类号： 文献标志码：
基金项目:国家高技术研究发展计划（2014AA022201-05）；山东省自然科学基金（ZR2014YL005）
引用文本：
津ICP备20000603号-1