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投稿时间：2017-03-15
投稿时间：2017-03-15
中文摘要: 以汉中桂花为原料，通过微波辅助提取法，以乙醇浓度、回流时间、回流温度及料液比等因素为变量，黄色素提取率为响应值，设计正交试验得到黄色素提取最佳工艺条件。试验结果表明，最佳提取工艺为乙醇浓度80%、回流时间80 min、回流温度80℃、料液比1∶20（g/mL）。在此条件下，桂花黄色素提取率可达18.42%。同时，抑菌试验证实，桂花黄色素对大肠杆菌、金黄色葡萄球菌、枯草杆菌、沙门氏菌均有较好的抑菌效果。对金黄色葡萄球菌的抑菌活性最为显著，其最低抑菌浓度（MIC）为1.0 mg/mL；对大肠杆菌、枯草杆菌、沙门杆菌的MIC均为1.5 mg/mL。此外，桂花黄色素对食品还具有一定的防腐功能。
Abstract:The extraction process of yellow pigment from Osmanthus fragrans assisted by microwave pretreatment was studied by single factor experiment which controlled the ethanol concentration，extraction time，extraction temperature and solid-to-liquid ratio.And based on single experiment，the orthogonal experiment showed the optimum extraction conditions were ethanol concentration 80%，extraction time 80 min，extraction temperature 80℃ and material to liquid ratio 1 ∶20（g/mL）.In addition，the results of antibacterial experiment showed that the yellow pigment had different inhibition effects on four kinds of bacteria，and inhibition ability on Staphylococcus aureus was the strongest.The MIC of Staphylococcus aureus was 1.0 mg/mL，and Escherichia coli，Bacillus subtilis and Salmonella was 1.5 mg/mL.
keywords: Osmanthus fragrans yellow pigment microwave-assisted pretreatment extraction antibacterial activity
文章编号：201721004 中图分类号： 文献标志码：
基金项目:国家自然科学基金（21373132）；陕西省教育厅2015年科学研究项目（15JK1141）
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