Abstract:Glycoprotein is a glycoconjugate in which a protein carries one or more carbohydrate chains covalently attached to its polypeptide backbone.Glycoprotein has emerged as an important class of bioactive natural products extracted from natural resources.The purification of glycoprotein has intimate relation with the research of glycoprotein structure and function analysis.Reviewing the methods of purification of glycoprotein，in addition，the further study of purification of glycoprotein was addressed to find a novel approach for purification of glycoprotein，aiming to provide a theory basis for furthering research of glycoprotein.