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食品研究与开发:2017,38(19):47-51
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微波辅助提取金花葵多糖工艺及体外抗氧化性研究
陈冯钱,黄杰涛,郑婷婷,张莲姬*
(延边大学理学院,吉林延吉133000)
Microwave-assisted Extraction and Antioxidant Activities of Polysaccharides from Aurea helianthustems Polysaccharide
CHEN Feng-qian,HUANG Jie-tao,ZHENG Ting-ting,ZHANG Lian-ji*
(College of Science,Yanbian University,Yanji 133000,Jilin,China)
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投稿时间:2017-02-06    
中文摘要: 以多糖得率为指标,采用正交设计法优化微波辅助提取金花葵多糖的工艺,并测定金花葵多糖的体外抗氧化活性。结果表明:微波辅助提取金花葵多糖的最佳工艺条件为微波功率280 W、微波辐射时间2 min、提取温度70℃、提取时间3.0 h,在此条件下多糖平均得率为5.51%;金花葵多糖对羟基自由基、超氧阴离子自由基具有明显的清除能力,并能够有效抑制小鼠肝匀浆脂质的过氧化,其IC50分别为0.068、1.23、1.20 mg/mL。
中文关键词: 金花葵  多糖  微波辅助提取  抗氧化
Abstract:Based on polysaccharide yield indicators,the optimum processing conditions of microwave-assisted extraction of polysaccharides fromAurea helianthustemswere studied by orthogonal design method,and determined the antioxidant activity ofAurea helianthustemspolysaccharide.The results indicated that the optimum extraction parameters of polysaccharide were microwave power 280 W,microwave radiation time 2 min,hydrothermal extraction temperature 70℃,hydrothermal extraction time 3.0 h.Under the optimum conditions,the average extraction rate of polysaccharide was 5.51%.Aurea helianthustemspolysaccharide had the effective scavenging activities of hydroxyl radical and superoxide anion free radical.In addition,it could inhibit the peroxidation of the mouse liver homogenate.The IC50was 0.068,1.23,1.20 mg/mL,respectively.
文章编号:201719011     中图分类号:    文献标志码:A
基金项目:延边大学2016年大学生创新创业训练计划项目(ydbksky2016101)
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