Abstract:In the process of making wine by sugarcane juice，the content of methanol and the clarity of wine could be ruduced by enzymolysis with pectinase.The optimum conditions for enzymatic hydrolysis of sugarcane juice were obtained through single factor and orthogonal experiment，and the content of D-galactose acid was used as the index.The result was showed as follows：the temperature and time of enzymatic hydrolysis was 60 ℃and 3.5 h respectivily，the pectinase dosage was 1.0%，the enzymatic hydrolysis of pH was 2.5，in these conditions the content of D-galacturonic acid in sugarcane juice was the highest of 85.5 mg/mL by enzymatic hydrolysis，the effect of enzymatic treatment was best.