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投稿时间:2016-12-16
投稿时间:2016-12-16
中文摘要: 为详细了解甜叶菊水提物和絮凝上清液的化学成分差异,建立超高效液相-串联四级杆飞行时间质谱(ultra-performance liquid chromatography quadrupole time of flight mass spectrometry,UPLC-QTOF-MS)分析方法并在此基础上对两种样品成分进行分析比较。研究结果表明,此方法可有效地将各种化合物分离开来,利用此方法检测比较得出,甜叶菊水提物和絮凝上清液化学成分差异显著,甜叶菊水提物中69种化合物经絮凝后上清液中只含有35种化合物。就去除种类而言,絮凝过程去除绿原酸类和去除黄酮类物质较为显著。
中文关键词: 超高效液相-串联四级杆飞行时间质谱 甜叶菊 水提物 絮凝上清液 化学成分
Abstract:An ultra-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLCQTOF-MS)method was developed for more information about the chemical composition differences of stevia extract and flocculation supernatant.The results indicated that the various compounds had been separated effectively by this method.There were 69,35 compounds which were detected in water extract and flocculation supernatant,respectively.The results also indicated that more chlorogenic acid and flavonoids could be removed than other components by flocculation process.
keywords: ultra-performance liquid chromatography quadrupole time of flight mass spectrometry(UPLCQTOF-MS) Stevia rebaudiana water extract flocculation supernatant chemical composition
文章编号:201718029 中图分类号: 文献标志码:A
基金项目:河北省重大科技成果转化专项(16047101Z)
作者 | 单位 |
额尔敦巴雅尔1,2,赵鹏宇1,2,焦利卫1,2,徐建中1,2,连运河1,2,* | 1.河北省天然色素工程技术研究中心,河北邯郸 057250;2.晨光生物科技集团股份有限公司,河北邯郸057250 |
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