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投稿时间:2016-12-21
投稿时间:2016-12-21
中文摘要: 为比较黄刺玫果醇提物不同极性萃取部分的体外抗氧化活性。以抗坏血酸为阳性对照,采用DPPH自由基清除法、邻苯三酚自氧化法和邻二氮菲亚铁法,测定果实提取物不同极性萃取部分对DPPH自由基(DPPH·)、超氧阴离子自由基(O2-·)、羟基自由基(·OH)的清除能力;采用亚硝酸钠-硝酸铝方法测定总黄酮含量,福林-酚方法测定多酚含量。结果表明:黄刺玫果醇提物的不同极性萃取物均有一定的抗氧化能力,并且在一定质量浓度范围内,清除自由基的能力与其质量浓度呈正相关性。其中乙酸乙酯部分对DPPH·、·OH、O2-·的清除能力最强,IC50分别是18.52、737.00、605.50 μg/mL。总黄酮含量和多酚含量测定结果表明,乙酸乙酯部分总黄酮含量和多酚含量最高,分别为15.51%和26.59%。不同极性部分中总黄酮和多酚所起的抗氧化作用相当(R2黄酮在0.98~0.37之间,R2多酚在0.98~0.37之间)。果实粗提物乙酸乙酯萃取部分是一种很有潜力的抗氧化剂,可以进一步开发利用为抗氧化保健食品。
Abstract:To study the antioxidant activity of the different polarities of ethanol extract of fruit from Rose xanthina Lindl.in vitro.The antioxidant activity was measured with the methods of DPPH·scavenging,pyrogallol autoxidation and phenanthroline by using ascorbic acid as the positive control.Measured total flavonoids in the ethyl acetate with the methods of Sodium nitrite-aluminium nitrate chromogenic,phenolic compounds determine by Foline-reagent method.The results showed that the extracts had some certain.antioxidant activity,and exhibited a concentration-dependent radical scavenging activity in a certain concentration range.Different ploarities had different ability of scavenging free radical,in which the ethyl acetate fraction of the fruit had the most potent free radical scavenging activity on DPPH·,O2-·,·OH,with IC5018.52,737.00,605.51 μg/mL,respectively.Furthermore the ratio of the total flavoroids in the ethyl acetate fraction of the fruit was 15.50%and the phenolic compounds was 26.59%.The flavonoids and polyphenols of different polarity had the same effect on antioxidant(R2黄酮and R2多酚were between 0.98-0.37).The ethyl acetate of the fruit is a potential natural antioxidant,It also can be further development and utilization of antioxidant functional food.
文章编号:201718003 中图分类号: 文献标志码:A
基金项目:山西省国际基金中美合作项目(2014081049-2)
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