Abstract:Goat milk is favored by consumers because of its good nutritional benefites， but it is becomig a main adulteration target. Multiplex Real-Time PCR（RT-PCR） is used for detecting ovine， bovine and plant derived components. According to the highly conserved gene sequences， the specific primers and Taqman probes were designed. Meanwhile， the stable duplex and triplex RT-PCR system were established for the detection of ovine， bovine and plant derived ingredients， and introduced into the specific test to guarantee the accuracy. The detec－ tion limit of this sysytem for DNA quality of ovine， bovine and plant derived components was 0.1 ng/μL respec－ tively. The volume adulterated detection limit of cow milk in goat milk was 0.1 %（volume fraction） in dairy mix－ tures； the detection limit of soy bean milk in goat milk was 0.5 %（volume fraction） in dairy mixtures.This multi－ plex fluorescent real time PCR assay had the advantages of accuracy， high sensitive and good specificity for de－ tecting the adulteration of goat milk products.