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投稿时间：2016-11-28
投稿时间：2016-11-28
中文摘要: 建立香草醛-盐酸法测定紫山药中原花青素含量的方法。主要从光照影响、反应时间、反应温度、香草醛用量 和盐酸浓度 5 个方面对试验条件进行优化，结果表明测定的最佳条件为：紫山药提取液 1 mL， 10 g/100 mL 香草醛溶 液 5 mL，浓盐酸 3 mL，而后用甲醇定容至 10 mL，在 20 ℃下静置 10 min，于 500 nm 处测定其吸光度。经测定该方法线 性范围为 4 μg/mL~24 μg/mL，相对标准偏差 RSD 为 1.10 %，回收率为 98.06 %~103.89 %，可以作为紫山药中原花青 素的测定方法。
Abstract:The vanillin method to determine the concentration of procyanidins in purple yam was established. The experimental conditions were optimized from five aspects： effect of illumination， reaction time， reaction temperature， the amount of vanillin and concentration of hydrochloric acid. The experimental results showed that when 1 mL extracting solution of purple yam was injected to volumetric flask， the optimal reaction condi－ tions were as the following： 5 mL of 10 g/100 mL vanillin solution， 3 mL of concentrated hydrochloric acid. Af－ ter diluted to 10 mL， the volumetric flask was placed in 20 ℃ for 10 minutes， and then the absorbance was de－ tected at 500 nm. According to the results， the line of range was from 4 μg/mL to 24 μg/mL， the relative stan－ dard deviation was 1.10 %， and the recoveries were from 98.06 % to 103.89 %. The results proved that this method could be used for the determination of proanthocyanidins in purple yam.
keywords: vanillin-HCl method procyanidins purple yam
文章编号：201715031 中图分类号： 文献标志码：A
基金项目:郑州市普通科技攻关项目（153PKJGG424）；2015 年度河南省高等学校优秀教学团队建设;2014年度河南省高等学校“专业综合改革试点”项目
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