Abstract:High performance liquid chromatography （HPLC） and liquid chromatography-mass spectrometry （LC-MS） for determination of the oligosaccharides in jujube were used. A method based on HPLC was estab－ lished for simultaneous determination of various saccharides in jujube， to determine the content of oligosaccha－ rides in different kinds of jujube. the samples were extracted by 70 % ethanol， the separation was achieved on a HPLC with Ca-column， the qualitative analysis was achieved on a LC-MS with cationic mode， the external calibration curves were used in quantification. Kestose and nistose trihydrate were identified by the retention values of HPLC and LC-MS. 5 kinds of main saccharides were satisfactorily separated by the established HPLC method， the linear range was 0.30 mg/mL-10.0 mg/mL （r = 0.999 6）， the relative standard deviations（RSD） of repeated measurement were between 0.53 %-0.83 %， standard addition recovery rate was between 97 % 108 %. By the method， we analyzed the content of jujube oligosaccharides from 32 different varieties， it turned out that the total content of 5 saccharides was between 223 mg/g F.W-395 mg/g F.W， the highest content of the jin si xin NO.1， the total content of 2 oligosaccharides was between 3.1 mg/g F.W-14.8 mg/g F.W， the highest content of the fuping jujube. Kestose and nistose trihydrate were the representative oligosaccharides in jujube， the content reached up to 1 % in the mature period.