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投稿时间：2016-11-12
投稿时间：2016-11-12
中文摘要: 以竹柳叶为原料，采用乙醇回流提取法对竹柳叶黄酮的提取工艺进行研究，确定最优提取工艺，并对黄酮类 成分进行初步分析。在单因素试验的基础上，以黄酮得率为指标，选取提取温度、料液比、乙醇浓度、提取时间 4 个因 素进行正交试验，对提取工艺进行优化。采用高效液相-离子阱-飞行时间质谱（LCMS-IT-TOF）技术对黄酮成分进行 分析。结果表明：竹柳叶黄酮的最佳提取工艺为提取温度 80℃、料液比 1 ∶ 40（g/mL）、乙醇浓度 55 %、提取时间 2 h，此 条件下，黄酮得率为 4.0 %。共推测出 6 种黄酮类化合物，即异牡荆素-7-O-葡萄糖苷、芦丁、木犀草素-7-O-葡萄糖 苷、异鼠李素-3-O-芸香糖苷、芹黄素-7-O-葡萄糖醛酸苷、金圣草素-葡萄糖醛酸苷。
Abstract:The ethanol refluxing extraction process of flavonoids from bamboo willow leaves was studied to de－ termine the optimal extraction conditions. The flavonoids were also analyzed.Based on the single-factor tests，the ethanol refluxing extraction conditions of flavonoids were optimized by orthogonal experiment concluding extrac－ tion temperature，liquid-solid ration，ethanolconcentr-ation and extraction time in order to increase the yield of flavonoids. The liquid chromatography-mass spectrometry-ion trap-time-of-flight （LCMS-IT-TOF）method was applied to analyze flavonoids from bamboo willow leaves. The reswlts showed that： The optimal extraction conditions of flavonoids were extraction temperature of 80 ℃，material-liquid ratio of 1 ∶ 40（g/mL），ethanol con－ centration of 55 % and extraction time of 2 h. Under the optimal conditions，the maximum yield of flavonoids was 4.0 %. Six flavonoids including isovitexin-7-O-glucoside，rutin，luteolin-7-O- glucoside， isorhamnetin-3-O-rutinoside，apigenin-7-O-glucuronide and chrysoeriol- glucuronide were speculated.
keywords: bamboo willow leaves flavonoids extraction analysis
文章编号：201714012 中图分类号： 文献标志码：A
基金项目:
| Author Name | Affiliation |
| HAN Ying， L譈 Xiao-ling | Institute of Food Engineering and Biotechnology， Tianjin University of Science and Technology， Tianjin 300457， China |
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