Abstract:In order to further explore the diversity of Saccharomyces cerevisiae of Helan Mountain Area in Ningxia and realize better development and utilization of Saccharomyces cerevisiae resources， the wine grape regions of Mausoleum of Xixia Dynasty， Hongsibu， Yuquanying， Qingtongxia and Chateau Changyu Moser XV were taken as the research object. The Saccharomycetes in the soil was isolated， screened and observed in the natural fermentation process of grape berries epidermis and the fruit. The 122 Saccharomycetes strainswere pre－ liminarily classified based on WL nutrient agar， and 26S rDNA D1/D2 sequence analysis was conducted. Total－ ly， 5 kinds of Saccharomycetes were identified in this study， including Pichia kluyveri， Metschnikowia pul－ cherrima， Hanseniaspora uvarum， Saccharomyces cerevisiae and Cryptococcus magnus. Thus the main species of Saccharomycetes in the wine grape region of Helan Mountain Area in Ningxia was preliminarily determined， which provides theoretical foundation and research basis for the screening and breeding of local Saccharomyces cerevisiae.