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投稿时间：2016-08-30
投稿时间：2016-08-30
中文摘要: 为提高右旋糖酐的产率，对肠膜明串珠菌 CICC-21725 发酵培养基进行优化。在单因素试验的基础上，根据 中心设计原理，采用响应面法确定培养基成分间交互作用及最优组成。结果表明：优化后各培养基成分为蔗糖 80.0 g/L， 蛋白质 7.0 g/L，磷酸氢二钠 1.5 g/L，磷酸二氢钾 0.3 g/L，在 pH7.1、25 ℃、150 r/min 时发酵 21 h，右旋糖酐产率可达92.79 %，与单因素优化后的右旋糖酐产率相比，提高了 7.2 %。红外光谱及核磁共振分析表明，发酵产物为右旋糖酐。
Abstract:In order to improve the yield of dextran， the fermentation midium of Leuconostoc Mesenteroides CI－CC-21725 had been optimized. On the basis of single factor experiments， according to the principle of design center， the response surface method was adopted to determine the interaction between medium composition and the optimal composition of medium composition. The medium optimized results indicated when sucrose， pep－ tone， disodium hydrogen phosphate and potassium dihydrogen phosphate were 80.0， 7.0， 1.5 g/L and 0.3 g/L， adjusted pH to 7.1， fermentation 21 h at 25 ℃ and 150 r/min， dextran yield could reach 92.79 %. The dextran yield increased 7.2 % compared with the best ruslt after single factor optimization. Infrared spectrum and nuclear magnetic resonance （NMR） analysis showed that the fermentation products was dextran. The dextran yield was also improved after the medium composition had optimized through the response surface method.
文章编号：201711038 中图分类号： 文献标志码：A
基金项目:广西高校人才小高地建设创新团队项目（桂教人[2011]47）；广西科技型中小企业技术创新资金（桂科转 13109067） ；广西民族大学广西化工研究院研究生联合培养基地专项资金（研究生联合培养机制改-2013 年 113000100030001）
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