Abstract:A method for simultaneous determination of 5 opium poppy husk alkaloids in broth sample was de－ veloped by ultra performance liquid chromatography -electrospray tandem mass spectrometry. Broth samples were extracted by 0.1 mol/L hydrochloric acid， then concentrated and purified by ProElut PXC solid-phase ex－ traction column， after that were eluted by 5 % ammonia-methanol.The separation was performed on a Thermo Hypersil Gold（100 mm×2.1 mm×1.9 μm）column，and detected by mass spectrometry using electrospray ioniza－ tion （ESI） in positive mode under multiple reaction monitoring mode.The recovery rates of the spiked sample was in the range of 71.8 %-112.5 % and the relative standard deviation （RSD） was between 4.1 % to 11.0 % （n=6）. The detection limits for morphine，codeine，thebaine，papaverine and narcotine were 1.2，0.8，0.4，0.05 μg/kg and 0.04 μg/kg.This method can be successfully applied to the simultaneous detection for 5 opium poppy husk alkaloids in broth.