Abstract:This paper optimized the amount of substance concentration of grape seed proanthocyanidins mea－ surement method to determine the colorimetric condition， a standard curve was established. The amount of sub－ stance concentration of proanthocyanidins measurement conditions， establish a standard curve， the standard curve range 0.055 μmol/mL-0.275 8 μmol/mL， the detection limit was 2.912 ×10-4 μmol/mL， the recoveries of 95 %-100 %， precision of RSD was 0.757 1 %； test verified by measuring different degree of polymerization of proanthocyanidins， the results confirmed that the method of high sensitivity and good reproducibility. The method was suitable for the analysis different DP of proanthocyanidins.