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食品研究与开发:2017,38(9):157-160
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肉及肉制品中牦牛源性成分的PCR鉴别
段庆梓,尚柯,张彪,张玉,王巍,梁恒兴*
(成都市食品药品检验研究院,四川 成都 610045)
PCR Identification of Yak in Meat and Meat Products
DUAN Qing-zi, SHANG Ke, ZHANG Biao, ZHANG Yu, WANG Wei, LIANG Heng-xing*
(Chengdu Institute of Food and Drug Control, Chengdu 610045,Sichuan,China)
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本文已被:浏览 1527次   下载 1052
投稿时间:2017-02-17    
中文摘要: 通过牦牛与其他常见物种的细胞色素 b 基因序列进行比对,设计牦牛的特异性引物,并进行聚合酶链式反应 (Polymerase Chain Reaction, PCR)体系中不同条件、参数的考察,建立肉及肉制品中牦牛源性检测的 PCR 方法。该方法具有较好的专属性,建立的 PCR 体系仅对牦牛源性有扩增现象,而对其他物种无扩增,且对牦牛源性的检出限可达 到 1 %。能够满足检验机构或生产企业对肉及肉制品中牦牛源性的检测需求。
Abstract:The specific primer of yak was designed by cytochrome b gene alignment between yak and other common species, which was used to establish the PCR detection of yak in meat products by Investigation of dif- ferent conditions and parameters. The established PCR system had better specificity, which could only have am- plification of yak, and not amplification of other species. The detection limit of yak could reach to 1 %. The yak PCR system established could meet the demand of institutions or production enterprises to detect the source of yak in meat and meat products.
文章编号:201709035     中图分类号:    文献标志码:A
基金项目:成都市科技惠民应用示范项目(2015-HM02-000990SF)
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